Selective inhibition of brain endothelial Rho-kinase-2 provides optimal protection of an in vitro blood-brain barrier from tissue-type plasminogenactivator and plasmin.
Rho-kinase (ROCK) inhibition, widely used in cardiovascular disease, could protect the blood-brain barrier (BBB) from damage during thrombolysis rt-PA-induced. While the use of inhibitors of ROCK nonselective as fasudil together with rt-PA can be deterred by the possibility of hypotension side effects and inadequate capacity to block the activity of rt-PA detrimental to endothelial cells of the brain (BEC), a selective ROCK-2 inhibition may overcome these limitations , Here, we examine ROCK-2 expression in primary brain cells and compare the ability of fasudil and KD 025, a selective ROCK-2 inhibitor, to attenuate the rt-PA-induced BBB disruption in vitro human models.
ROCK-2 highly expressed relative to ROCK-1 in all kinds of human and mouse brain cells and particularly enriched in rat brain endothelial cells and astrocytes compared to neurons. KD 025 is stronger than fasudil in attenuation of rt-PA-induced plasminogen-and permeation BBB under normoxia, but especially under conditions such as stroke. Importantly, only KD 025, but not fasudil, was able to block the increase in the permeability of rt-PA-dependent, changes in morphology and tight junction degradation in BECs isolated.
Selective inhibition of ROCK-2 further diminished rt-PA-induced phosphorylation of myosin, transformation and activation of matrix metalloprotease in astrocytes. These findings highlight the isoforms ROCK-2 as the main driving BBB endothelial disorders and brain damage by rt-PA and the potential of KD 025 to optimally protect the BBB during thrombolysis.
Selective inhibition of brain endothelial Rho-kinase-2 provides optimal protection of an in vitro blood-brain barrier from tissue-type plasminogenactivator and plasmin.
Evaluation fibrinolytic Inhibitors : alpha- two -Antiplasmin and Plasminogen Activator inhibitors 1 patients with Obstructive Sleep Apnea.
Obstructive sleep apnea (OSA) inducing thrombophilia and reduced fibrinolysis. Alpha-2-antiplasmin (a-2-AP) and plasminogen activator inhibitor 1 (PAI-1) is the main inhibitor of the fibrinolytic system. Increasing concentrations of this factor is associated with a higher risk of cardiovascular disease. The purpose of this study was to evaluate plasma-2-AP and PAI-1 in patients with OSA and evaluate the correlation with polysomnographic records and selected risk factors of cardiovascular disease.
The study group comprised 45 patients with OSA, and the control group consisted of 19 patients who did not meet the diagnostic criteria of OSA. Plasma-2-AP and PAI-1 concentrations assessed by enzyme-linked immunosorbent assay (ELISA). In the study group, the median value of plasma-2-AP is higher than the control group (11.89 vs 157.34 pg / ml, respectively, P <0.0001). A-2-AP concentration increases proportionally with the severity of OSA.
The concentration of 2-AP positively correlated with the index of apnea-hypopnoea (AHI), apnea index (AI), respiratory disturbance time (RDT), and the index desaturaion (DI), and negatively correlated with oxygen saturation, mean and minimum (SpO2 means, SpO2 min, respectively). The median value of PAI-1 was higher in the study group than the control group (12.55 vs. 5.40 ng / ml, respectively, P = 0.006) and increased with the severity of OSA. PAI-1 concentrations correlated positively with AHI, AI, RDT, DI, and body mass index (BMI) and negatively correlated with mean SpO2 and SpO2 min.
Higher plasma concentrations of 2-AP and PAI-1 in patients with OSA showed that these patients had increased prothrombotic activity. OSA increases the risk of cardiovascular complications such as increased prothrombotic activity.
Description: SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 amino acids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
Description: SERPINE1 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (24-402) and having a molecular mass of 45 kDa.;The SERPINE1 is purified by proprietary chromatographic techniques.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Plasminogen Activator Inhibitor 2 (PAI2) Protein
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Mouse Plasminogen activator inhibitor 2 (SERPINB2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Plasminogen activator inhibitor 2(SERPINB2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Mouse Plasminogen Activator Inhibitor 1 (PAI1) Protein
Description: A sandwich CLIA kit for quantitative measurement of Mouse PAI-2 (Plasminogen Activator Inhibitor 2) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse PAI2 (Plasminogen Activator Inhibitor 2)
Description: A sandwich ELISA kit for quantitative measurement of Mouse PAI2 (Plasminogen Activator Inhibitor 2) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse PAI2 (Plasminogen Activator Inhibitor 2)
Description: A sandwich ELISA kit for detection of Plasminogen Activator Inhibitor 2 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Plasminogen activator inhibitor 1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in samples from serum, plasma or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Plasminogen Activator Inhibitor 1 (PAI1) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Canine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
PAI-2 ELISA Kit| Mouse Plasminogen Activator Inhibitor 2 ELISA K
Description: A competitive ELISA for quantitative measurement of Goat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Mouse plasminogen activator inhibitor 1, PAI-1 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse plasminogen activator inhibitor 1, PAI-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor 1,PAI-1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor 1,PAI-1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor 1,PAI-1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA kit for quantitative measurement of Mouse PAI1 (Plasminogen Activator Inhibitor 1) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse PAI1 (Plasminogen Activator Inhibitor 1)
Description: A sandwich ELISA kit for detection of Plasminogen Activator Inhibitor 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA for quantitative measurement of Rat Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Plasminogen Activator Inhibitor 2 (PAI2) Protein
Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator Inhibitor 2 (PAI2). This antibody is labeled with PE.
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A significant effect of rs2070682 in the risk of early onset DR found in codominant model of inheritance [odds ratio, OR (95% confidence interval, CI): 5:04 (1.47 to 17.28), p = 0.018]. However, this association did not survive some correction bit testing. No other significant associations between PAI-1 tag SNPs and haplotypes revealed. In addition, no significant effect of the mutation load of PAI-1 tag SNPs on the risk of developing type 2 diabetes found in the course DR.
