Selective inhibition of brain endothelial Rho-kinase-2 provides optimal protection of an in vitro blood-brain barrier from tissue-type plasminogenactivator and plasmin.
Rho-kinase (ROCK) inhibition, widely used in cardiovascular disease, could protect the blood-brain barrier (BBB) from damage during thrombolysis rt-PA-induced. While the use of inhibitors of ROCK nonselective as fasudil together with rt-PA can be deterred by the possibility of hypotension side effects and inadequate capacity to block the activity of rt-PA detrimental to endothelial cells of the brain (BEC), a selective ROCK-2 inhibition may overcome these limitations , Here, we examine ROCK-2 expression in primary brain cells and compare the ability of fasudil and KD 025, a selective ROCK-2 inhibitor, to attenuate the rt-PA-induced BBB disruption in vitro human models.
ROCK-2 highly expressed relative to ROCK-1 in all kinds of human and mouse brain cells and particularly enriched in rat brain endothelial cells and astrocytes compared to neurons. KD 025 is stronger than fasudil in attenuation of rt-PA-induced plasminogen-and permeation BBB under normoxia, but especially under conditions such as stroke. Importantly, only KD 025, but not fasudil, was able to block the increase in the permeability of rt-PA-dependent, changes in morphology and tight junction degradation in BECs isolated.
Selective inhibition of ROCK-2 further diminished rt-PA-induced phosphorylation of myosin, transformation and activation of matrix metalloprotease in astrocytes. These findings highlight the isoforms ROCK-2 as the main driving BBB endothelial disorders and brain damage by rt-PA and the potential of KD 025 to optimally protect the BBB during thrombolysis.
Evaluation fibrinolytic Inhibitors : alpha- two -Antiplasmin and Plasminogen Activator inhibitors 1 patients with Obstructive Sleep Apnea.
Obstructive sleep apnea (OSA) inducing thrombophilia and reduced fibrinolysis. Alpha-2-antiplasmin (a-2-AP) and plasminogen activator inhibitor 1 (PAI-1) is the main inhibitor of the fibrinolytic system. Increasing concentrations of this factor is associated with a higher risk of cardiovascular disease. The purpose of this study was to evaluate plasma-2-AP and PAI-1 in patients with OSA and evaluate the correlation with polysomnographic records and selected risk factors of cardiovascular disease.
The study group comprised 45 patients with OSA, and the control group consisted of 19 patients who did not meet the diagnostic criteria of OSA. Plasma-2-AP and PAI-1 concentrations assessed by enzyme-linked immunosorbent assay (ELISA). In the study group, the median value of plasma-2-AP is higher than the control group (11.89 vs 157.34 pg / ml, respectively, P <0.0001). A-2-AP concentration increases proportionally with the severity of OSA.
The concentration of 2-AP positively correlated with the index of apnea-hypopnoea (AHI), apnea index (AI), respiratory disturbance time (RDT), and the index desaturaion (DI), and negatively correlated with oxygen saturation, mean and minimum (SpO2 means, SpO2 min, respectively). The median value of PAI-1 was higher in the study group than the control group (12.55 vs. 5.40 ng / ml, respectively, P = 0.006) and increased with the severity of OSA. PAI-1 concentrations correlated positively with AHI, AI, RDT, DI, and body mass index (BMI) and negatively correlated with mean SpO2 and SpO2 min.
Higher plasma concentrations of 2-AP and PAI-1 in patients with OSA showed that these patients had increased prothrombotic activity. OSA increases the risk of cardiovascular complications such as increased prothrombotic activity.
Description: SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 amino acids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
Description: SERPINE1 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (24-402) and having a molecular mass of 45 kDa.;The SERPINE1 is purified by proprietary chromatographic techniques.
Recombinant Human Plasminogen activator inhibitor 1 (SERPINE1), partial
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Mouse Plasminogen activator inhibitor 2 (SERPINB2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Plasminogen activator inhibitor 2(SERPINB2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
A significant effect of rs2070682 in the risk of early onset DR found in codominant model of inheritance [odds ratio, OR (95% confidence interval, CI): 5:04 (1.47 to 17.28), p = 0.018]. However, this association did not survive some correction bit testing. No other significant associations between PAI-1 tag SNPs and haplotypes revealed. In addition, no significant effect of the mutation load of PAI-1 tag SNPs on the risk of developing type 2 diabetes found in the course DR.