Hereditary angioedema with normal levels of C1 inhibitor (HAE-N) is associated with mutations in Factor XII in 30% of subjects; However, the role of this mutation in the pathogenesis of angioedema is unclear. We are looking for evidence of abnormalities in the pathway of bradykinin bradykinin formation and degradation in the plasma of patients with HAE-N both with and without the mutation.
Bradykinin has been added to the plasma, and the degradation rate was measured using ELISA. autoactivation Plasma assessed using a chromogenic assay kallikrein formation. Plasminogen activator inhibitor (Pais) 1 and 2 were measured by ELISA method.
PAI-1 levels varied from 0.1 to 4.5 ng / mL (median, 2.4 ng / mL) in 23 control subjects, 0.0 to 2 ng / mL (mean, 0.54 ng / ml) in patients with HAE-N with factor XII mutations (12 samples), and 0.0 to 3.7 ng / mL (mean, 1.03 ng / mL) in patients with HAE-N without factor XII mutation (11 samples). PAI-2 levels vary 25-87 ng / mL (median, 53.8 ng / mL) in control subjects and 0-25 ng / mL (median, 4.3 ng / mL) in patients with HAE- N with or without the mutation Factor XII. Autoactivation at 1: 2 dilution is abnormally high in 8 of 17 patients with HAE-N (4 in each sub-category) and can be corrected with additional C1 inhibitor in 4 of them. bradykinin degradation is real abnormal in 1 out of 23 patients with HAE-N and normal in the remaining 22 patients.
bradykinin degradation is normal in all but one of 23 patients with HAE-N studied. Conversely, there are disorders characterized in PAI-2 levels in patients with HAE-N were not seen in patients with C1 inhibitor deficiency. PAI-1 levels vary, but the difference was not statistically significant visible. A link between excessive fibrinolysis and bradykinin generation suggested that estrogen dependent.
Copper (II) ions Increases Plasminogen Activator Inhibitor Type 1 Structural Dynamics in Key Regions Memerintah Stability.
Plasminogen activator inhibitor type 1 (PAI-1) regulates the fibrinolysis pathway by inhibiting the protease activity of plasminogen activators. PAI-1 works in concert with vitronectin (VN), an extracellular protein that aids in localization of active PAI-1 to the network. Peterson laboratory showed that Cu (II) and other transition metals modulate the stability of PAI-1, the effect of which depends on the presence or absence of somatomedin B (SMB) domain of VN show.
The studies presented here dissect the molecular changes underlying dynamics destabilizing effect of Cu (II) on PAI-1. We use exchange backbone amide hydrogen / deuterium monitored by mass spectrometry to assess the dynamics of PAI-1 in the presence and absence of Cu (II) ion with and without the SMB domain of VN. We show that Cu (II) results in improved dynamics in areas essential to the function and the overall stability of PAI-1, whereas the SMB domain elicits almost the opposite effect.
A mutant form of PAI-1 less two histidine residues N-terminal in position 2 and 3 exhibit a similar rise in the dynamics of the Cu (II) binding compared to an active wild type PAI-1, suggesting that the structural effect observed is not the result of coordination of Cu ( II) to the histidine residues.
Description: SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 amino acids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
Description: SERPINE1 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (24-402) and having a molecular mass of 45 kDa.;The SERPINE1 is purified by proprietary chromatographic techniques.
Recombinant Human Plasminogen activator inhibitor 1 (SERPINE1), partial
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen activator inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in plasma.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Plasminogen Activator Inhibitor 2 (PAI2) in samples from plasma with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Β± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Β± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Plasminogen Activator Inhibitor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse plasminogen activator inhibitor,PAI in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Finally, the addition of Cu (II) produces an acceleration of the kinetics of local unfolding of PAI-1 are thought to be on the path to conversion latency. The influence of the ligand on the dynamics of PAI-1 adds another interesting dimension to the mechanism of regulation of PAI-1 stability and function.