Elevated Expression of Vascular Adhesion Molecule-1, Plasminogen Activator Inhibitor-1, Cyclooxygenase-2, and Thrombomodulin in Human Umbilical Vein Endothelial Cells from Hospitalized Gestational Diabetes Mellitus Patients.
protein expression in human umbilical vein endothelial cells (HUVECs) is a useful indicator of the condition of the mother during pregnancy and the intrauterine environment. Therefore, we examined the protein expression in HUVECs were obtained from patients with gestational diabetes mellitus (GDM). HUVECs were made from the umbilical cord and control GDM patients undergoing planned caesarean section between 2013 and 2014 at Teikyo University Hospital (Tokyo, Japan).
There was no difference in blood glucose levels between patients with GDM and control the time of entry. However, body mass index, pre-pregnancy (BMI) were higher in GDM patients, although changes in pregnancy BMI smaller during hospitalization. To evaluate the state of the endothelium, we examined the protein expression levels of vascular adhesion molecule-1 (VCAM-1), an adhesion molecule-1, thrombomodulin (TM), endothelial nitric oxide synthase, plasminogen activator inhibitor-1 (PAI- 1), siklooksigenase- 2 (COX-2), and VE-cadherin, which is changed by various factors in the endothelial tissue.
VCAM-1, PAI-1 and COX-2 expression was higher in HUVECs of patients with GDM compared to controls. Due to pre-pregnancy BMI was higher in patients with GDM, we examined the relationship between BMI and protein expression. However, the protein expression levels are not correlated with pre-pregnancy BMI and higher in HUVECs of GDM patients than other BMI of BMI-matched controls.
Interestingly, the expression of TM was also higher in HUVECs of BMI-matched patients with GDM. Thus, the expression of VCAM-1, PAI-1, COX-2, and TM may reflect certain factors are altered intrauterine environment in GDM patients hospitalized with weight under control.

Plasminogen driving inhibitors – two the development of excess bladder cancer support ( two PAI- ) in PAI-1 knockout mice in N-butyl-N- (4-hydroxybutyl) -nitrosamine- induced rat model of bladder cancer.
Collecting evidence suggests that the plasminogen activator inhibitor-1 (PAI-1) play an important role in bladder tumorigenesis by regulating the cell cycle. However, it remains unclear whether and how the inhibition of PAI-1 Pressing bladder tumorigenesis.To explain the therapeutic effect of PAI-1 inhibition, we tested the tumorigenicity in PAI-1 knockout (KO) mice exposed to bladder known carcinogen.PAI- 1 deficiency is not inhibit carcinogen-induced bladder cancer in carcinogen-exposed rats although wild-type mice significantly increased PAI-1 levels in bladder tissue, plasma and urine.
We found that PAI-1 KO mice exposed to carcinogens tend to upregulate protein C inhibitor (PAI-3), urokinase-type plasminogen activator (uPA) and tissue-type PA (TPA), and significantly increased PAI-2, indicating the potential compensation function of these molecules when PAI-1 was canceled. subsequent studies using gene expression microarray using bladder tissue of mice followed by post hoc bioinformatics analysis and validation experiments by qPCR and IHC showed that SERPING1 subsequently downregulated in PAI-1 KO mice exposed to BBN, suggesting that SERPING1 as a potential factor is missing which regulate PAI – 2 excess (track compensation) .
Aquaphile Coelenterazine h, lyophilized solid |
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9-10127 | Biotium |
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Description: Minimum order quantity: 1 unit of 50uG |
CF568 goat anti-mouse IgG(H+L) |
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9-20100 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20101 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 goat anti-rabbit IgG(H+L) |
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9-20102 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20103 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20104 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20105 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20106 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 rabbit anti-goat IgG(H+L) |
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9-20107 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 goat anti-guinea pig IgG(H+L) |
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9-20108 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-mouse IgG(H+L) |
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9-20110 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20111 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-rabbit IgG(H+L) |
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9-20112 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20113 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20114 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20115 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20116 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-goat IgG(H+L) |
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9-20117 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-guinea pig IgG(H+L) |
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9-20118 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-mouse IgG(H+L) |
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9-20120 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20121 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-rabbit IgG(H+L) |
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9-20122 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20123 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20124 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20125 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20126 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20127 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 rabbit anti-goat IgG(H+L) |
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9-20128 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-guinea pig IgG(H+L) |
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9-20129 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-human IgG(H+L), highly cross-adsorbed |
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9-20132 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-rat IgG(H+L), highly cross-adsorbed |
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9-20133 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20134 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 rabbit anti-rat IgG(H+L), highly cross-adsorbed |
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9-20135 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20136 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-rat IgG(H+L), highly cross-adsorbed |
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9-20137 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 goat anti-swine IgG(H+L) |
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9-20138 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-mouse IgG(H+L) |
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9-20140 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-rabbit IgG(H+L) |
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9-20141 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20142 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20143 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20144 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-rat IgG(H+L), highly cross-adsorbed |
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9-20147 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 donkey anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20148 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20149 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20152 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-human IgG(H+L), highly cross-adsorbed |
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9-20154 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-rat IgG(H+L), highly cross-adsorbed |
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9-20155 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20156 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-rat IgG(H+L), highly cross-adsorbed |
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9-20157 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20158 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-rat IgG(H+L), highly cross-adsorbed |
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9-20159 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 goat anti-swine IgG(H+L) |
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9-20160 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-chicken IgY ( IgG) (H+L) |
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9-20164 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 rabbit anti-chicken IgY (IgG) (H+L) |
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9-20165 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20166 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20167 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20168 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A donkey anti-guinea pig IgG(H+L), highly cross-adsorbed |
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9-20169 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 donkey anti-guinea pig IgG(H+L), highly cross-adsorbed |
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9-20170 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 donkey anti-guinea pig IgG(H+L), highly cross-adsorbed |
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9-20171 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A rabbit anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20172 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20173 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 rabbit anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20174 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20175 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20176 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20177 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R donkey anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20178 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20179 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF405M goat anti-mouse IgG(H+L) |
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9-20180 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF405M goat anti-rabbit IgG(H+L) |
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9-20181 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF405M goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20182 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin goat anti-mouse IgG(H+L) |
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9-20183 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20184 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin goat anti-rabbit IgG(H+L) |
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9-20185 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20186 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin donkey anti-goat IgG(H+L), highly cross-adsorbed |
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9-20189 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20190 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
Biotin donkey anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20191 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R goat anti-mouse IgG(H+L) |
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9-20197 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 goat anti-guinea pig IgG(H+L) |
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9-20198 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R donkey anti-rat IgG(H+L), highly cross-adsorbed |
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9-20199 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R rabbit anti-mouseIgG(H+L), highly cross-adsorbed |
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9-20200 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R rabbit anti-rat IgG(H+L), highly cross-adsorbed |
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9-20201 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R goat anti-rabbit IgG(H+L) |
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9-20202 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A chicken anti-mouse IgG(H+L) |
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9-20208 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A chicken anti-rabbit IgG(H+L) |
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9-20209 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 chicken anti-mouse IgG(H+L) |
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9-20221 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 chicken anti-mouse IgG(H+L) |
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9-20222 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 chicken anti-rabbit IgG(H+L) |
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9-20223 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 chicken anti-rabbit IgG(H+L) |
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9-20224 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A chicken anti-goat IgG(H+L) |
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9-20225 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 chicken anti-goat IgG(H+L) |
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9-20226 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 chicken anti-goat IgG(H+L) |
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9-20227 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 goat anti-human IgG(H+L), highly cross-adsorbed |
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9-20230 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20231 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20232 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 goat anti-rat IgG(H+L), highly cross-adsorbed |
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9-20233 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 donkey anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20234 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20235 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 goat anti-swine IgG(H+L) |
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9-20236 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF680 rabbit anti-guinea pig IgG(H+L) |
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9-20243 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF770 rabbit anti-guinea pig IgG(H+L) |
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9-20244 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF350 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20275 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF555 donkey anti-guinea pig IgG (H+L), highly cross-adsorbed |
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9-20276 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 goat anti-human IgG(H+L), highly cross-adsorbed |
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9-20280 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 goat anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20281 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 goat anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20282 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 goat anti-rat IgG(H+L), highly cross-adsorbed |
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9-20283 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 donkey anti-sheep IgG(H+L), highly cross-adsorbed |
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9-20284 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20285 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF647 goat anti-swine IgG(H+L) |
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9-20286 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed |
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9-20293 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 Bovine anti-goat IgG(H+L), highly cross-adsorbed |
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9-20294 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 Bovine anti-goat IgG(H+L), highly cross-adsorbed |
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9-20295 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF633 Bovine anti-goat IgG(H+L), highly cross-adsorbed |
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9-20296 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed |
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9-20297 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 Goat anti-mouse IgG (H+L), highly cross-adsorbed |
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9-20299 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 Goat anti-rabbit IgG (H+L), highly cross-adsorbed |
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9-20300 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat) |
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9-20301 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat) |
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9-20302 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat) |
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9-20303 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat) |
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9-20304 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 Donkey anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20305 | Biotium |
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 goat anti-mouse IgG(H+L) |
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9-20306 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 Rabbit anti-mouse IgG(H+L), highly cross-adsorbed |
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9-20307 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 Donkey anti-rabbit IgG(H+L), highly cross-adsorbed |
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9-20308 | Biotium |
|
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Description: Minimum order quantity: 1 unit of 1mg |
CF543 goat anti-rabbit IgG(H+L) |
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9-20309 | Biotium |
|
|
Description: Minimum order quantity: 1 unit of 1mg |
CF543 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed |
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9-20310 | Biotium |
|
|
Description: Minimum order quantity: 1 unit of 1mg |
This result indicates that the compensation serpin lanes, particularly PAI-2 overproduction in this model, support the development of bladder cancer when oncoprotein PAI-1 is deleted. Further investigation into the PAI-1 is required to identify the true potential targets for therapy of bladder cancer.